Method for the purification, recovery, and sporulation of...

C - Chemistry – Metallurgy – 12 – N

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C12N 1/10 (2006.01) A61K 39/002 (2006.01) A61P 31/00 (2006.01) C12N 3/00 (2006.01)

Patent

CA 2366896

A vaccine for in ovo vaccination against avian coccidiosis produced by a method including obtaining the coccidial oocysts from a fecal suspension, homogenizing the fecal suspension, separating the oocysts from the fecal debris by either salt flotation using sodium sulfate or gas flotation using air, sporulating the oocysts using hydrogen peroxide and air sparging, bleaching the sporulated oocysts, washing the bleached oocysts, concentrating the sterile washed oocysts and combining the concentrates of various species of coccidial oocysts, and producing a vaccine. The method in whole or in part can be applied to other kinds of encysted protozoa to produce vaccines for various types of animals.

L'invention concerne un vaccin destiné à la vaccination in ovo contre les coccidies aviaires, produit par une méthode consistant à obtenir des oocystes de coccidies à partir d'une suspension fécale, à homogénéiser ladite suspension, à séparer les oocystes des débris fécaux par flottation saline en utilisant du sulfate de sodium ou par flottation gazeuse à l'air, à sporuler les oocystes au moyen de peroxyde d'hydrogène et d'un barbotage d'air, à blanchir les oocystes sporulés, à laver les oocystes blanchis, à concentrer les oocystes lavés stérilisés et à combiner les concentrés de diverses espèces d'oocystes de coccidies, et à produire un vaccin. Cette méthode peut être appliquée entièrement ou en partie à d'autres genres de protozoaires enkystés afin de produire des vaccins pour divers animaux.

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