C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 14/47 (2006.01) C07K 16/18 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01) G01N 33/68 (2006.01)
Patent
CA 2227537
A method for the quantitative determination of human acute phase serum amyloid A protein (A-SAA) comprises contacting a sample of a biological fluid with antibody specific for A-SAA, the sample being reacted with an organic solvent prior to or simultaneous with antibody contact. The organic solvent is suitably a C1-C4 alcohol and the antibody can be used on the solid phase and as a component of the detection system of an enzyme linked immunosorbant assay. The method provides a sensitive and reliable measure of A-SAA and inflammatory status which can be used for the diagnosis and clinical management of both acute and chronic inflammatory conditions.
Procédé de détermination quantitative de la protéine amyloïde sérique A de phase aiguë (A-SAA) humaine, consistant à mettre un échantillon de liquide biologique en contact avec un anticorps spécifique de l'A-SAA, après l'avoir fait réagir avec un solvant organique, ou en même temps que cette réaction. Le solvant organique est avantageusement un alcool C¿1-4?, et l'anticorps peut s'utiliser sur la phase solide et comme élément du système de détection d'un test ELISA. Ce procédé permet d'obtenir une mesure sensible et fiable de l'A-SAA et de l'état inflammatoire, mesure que l'on peut utiliser dans le diagnostic et le traitement clinique des pathologies inflammatoires tant aiguës que chroniques.
Doyle John Martin
Hobson Hazel Audrey
Whitehead Alexander Steven
A. Body Incorporated By Charte R. The Provost Fellows And Scholars Of The College Of The Holy And
Bereskin & Parr
The Provost Fellows And Scholars Of The College Of The Holy And Undivid Ed T
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