Method for the quantitative measurement of human acute phase...

C - Chemistry – Metallurgy – 07 – K

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C07K 14/47 (2006.01) C07K 16/18 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2227537

A method for the quantitative determination of human acute phase serum amyloid A protein (A-SAA) comprises contacting a sample of a biological fluid with antibody specific for A-SAA, the sample being reacted with an organic solvent prior to or simultaneous with antibody contact. The organic solvent is suitably a C1-C4 alcohol and the antibody can be used on the solid phase and as a component of the detection system of an enzyme linked immunosorbant assay. The method provides a sensitive and reliable measure of A-SAA and inflammatory status which can be used for the diagnosis and clinical management of both acute and chronic inflammatory conditions.

Procédé de détermination quantitative de la protéine amyloïde sérique A de phase aiguë (A-SAA) humaine, consistant à mettre un échantillon de liquide biologique en contact avec un anticorps spécifique de l'A-SAA, après l'avoir fait réagir avec un solvant organique, ou en même temps que cette réaction. Le solvant organique est avantageusement un alcool C¿1-4?, et l'anticorps peut s'utiliser sur la phase solide et comme élément du système de détection d'un test ELISA. Ce procédé permet d'obtenir une mesure sensible et fiable de l'A-SAA et de l'état inflammatoire, mesure que l'on peut utiliser dans le diagnostic et le traitement clinique des pathologies inflammatoires tant aiguës que chroniques.

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