Method for the random diversification of a genetic sequence...

Details Method for the random diversification of a genetic sequence... Method for the random diversification of a genetic sequence...

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C40B 10/00 (2006.01)

Patent

CA 2673004

The invention relates to a very general method for the random diversification of a nucleotide sequence S by PCR while preserving the identity of some domains of said sequence S; the invention also relates to a bank of nucleotide sequence thus diversified, and to diversified proteins obtained by the expressions of the nucleotide sequences in an appropriate host.

La présente invention vise un procédé très général pour diversifier de manière aléatoire une séquence nucléotidique S par PCR, tout en préservant l'identité de certains domaines de ladite séquence S, une banque de séquences nucléotidiques ainsi diversifiées, les protéines diversifiées obtenues par expressions de ces séquences nucléotidiques dans un hôte approprié.

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