Method for the recombinant production of hirudins and...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/15 (2006.01) A61K 38/58 (2006.01) C07K 14/815 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 15/62 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2063575

2063575 9117250 PCTABS00008 A hirudin or hirudin-like polypeptide having anti-thrombin activity is prepared by recombinant DNA methodologies. The hirudin or hirudin-like polypeptide is preferably expressed in E. coli or insect cells. The hirudin-like polypeptide is preferably a hybrid protein from HV1 and HV2 having the following amino acid sequence: HV12: Val-Val-Tyr-Thr-Asp-Cys-Thr-Glu-Ser-Gly-Gln-Asn-Leu-Cys-Leu-Cys-Glu-Gly-Ser-A_ sn-Val-Cys-Gly-Gln-Gly-Asn-Lys-Cys-Ile-Leu-Gly-Ser-Asp-Gly-Glu-Lys-Asn-Gln-Cys_ -Val-Thr-Gly-Glu-Gly-Thr-Pro-Asn-Pro-Glu-Ser-His-Asn-Asn-Gly-Asp-Phe-Glu-Glu-I_ le-Pro-Glu-Glu-Tyr-Leu-Gln where the underlined sequence is the HV2 portion.

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