Method for the recovery of cellular proteins in active form

Details Method for the recovery of cellular proteins in active form Method for the recovery of cellular proteins in active form

C - Chemistry, Metallurgy

12

N

195/72, 530/3.12

C12N 1/06 (2006.01) C07K 1/113 (2006.01) C07K 14/61 (2006.01)

Patent

CA 1305284

A B S T R A C T A method for recovery of cellular proteins comprises treating host cells which contain the protein with at least one cationic anionic or zwitterionic surfactant and optionally a source of at least one polar organic solvent in an amount sufficient to effect cell lysis and protein solubilization without substantial modification to the structural break down of the protein. The treatment solution with the polar organic solvent present is a mixture of from approximately five to seventy percent volumes/volume of the polar organic solvent and the at least one surfactant. The solubilized protein is then separated from the resulting mixture. This process is particularly suitable for recovering monomeric and polymeric intra-cellular proteins, such as those found in cellular inclusion bodies and cyto-plasmic aggregates. Such proteins include growth hormones, interferons, immunogens and lymphokines.

569448

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