C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01)
Patent
CA 2495885
The present invention provides a method for the selective combinatorial randomization (SCR) of polynucleotides at specific sites which comprises providing a double stranded polynucleotide sequence having at least one differing site and selectively randomizing the polynucleotide at or in the proximity to the differing sites without the need for a determination of the sequence position of the differing site.
L'invention concerne un procédé de répartition aléatoire combinatoire et sélective (SCR) de polynucléotides sur des sites spécifiques. Ce procédé consiste à préparer une séquence polynucléotidique à double brin, dotée d'au moins un site différent, et à répartir de manière aléatoire et sélective le polynucléotide sur les sites différents ou à proximité de ceux-ci, sans avoir à déterminer la position séquentielle du site différent.
Kettling Ulrich
Koltermann Andre
Pilling Jens
Spangenberg Oliver
Bayer Schering Pharma Aktiengesellschaft
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Direvo Biotech Ag
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