C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2093647
The present invention relates to a method for the specific detection of a nucleic acid A in a sample which comprises the following steps in the given sequence: a) reacting the sample with one or several labelled mononucleoside triphosphates and one or several enzymes which catalyze the production of a labelled nucleic acid B which contains said labelled mononucleoside triphosphates, b) non-thermal denaturation of the reaction mixture subsequent to step a), c) hybridization of the nucleic acid B with a nucleic acid probe C which contains at least one immobilizable group and which is sufficiently complementary to the nucleic acid B, and simultaneous immobilization of the nucleic acid probe C to a solid phase which can bind the immobilizable nucleic acid probe C, d) separating the liquid phase from the solid phase, and e) detecting the nucleic acid hybrid D formed from the labelled nucleic acid B and nucleic acid probe C by detecting the detectable group bound to the solid phase.
Berner Sibylle
Kessler Christoph
Kruse-Mueller Cornelia
Rueger Ruediger
Seibl Rudolf
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
Roche Diagnostics Gmbh
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