Method for the separation of recombinant pro-factor ix from...

Details Method for the separation of recombinant pro-factor ix from... Method for the separation of recombinant pro-factor ix from...

C - Chemistry, Metallurgy

12

N

C12N 9/64 (2006.01) A61K 35/14 (2006.01) A61K 38/48 (2006.01) C07K 1/18 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2159700

A method for separation of recombinant pro-Factor IX from recombinant Factor IX by a chromatographic method is described. According to a preferred embodiment, the mixture of both proteins is bound to an ion exchanger and pro-Factor IX and Factor IX are eluted separately from each other by buffer solutions with different salt concentrations and/or pH values. According to the method of the invention, pro-Factor IX and Factor IX are obtainable in highly pure form and free from the other factor respectively.

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