Method for the supply of growth components to cell cultures

Details Method for the supply of growth components to cell cultures Method for the supply of growth components to cell cultures

C - Chemistry, Metallurgy

12

N

C12N 1/20 (2006.01)

Patent

CA 2739932

The present invention provides a method for improving the preparation and use of growth media by the uses of specific pellet formulations, which especially are tablets of different sizes, which contain the growth medium or parts thereof and are sterilized with the standard methods of pharmaceutical technology. Specifically these pellet formulations are applied to control a cell culture in a way that the adaptation phase is shorter or that the growth is controlled by a release of certain components at a certain time and in a certain concentration during the process, and nutrients (e.g., nitrogen) can be packed into the cultivation vessel in amounts sufficient for high cell densities without the risk of intoxication of the organism.

La présente invention porte sur un procédé d'amélioration de la préparation et de l'utilisation de milieux de croissance par les utilisations de formulations de pastilles spécifiques, qui sont notamment des comprimés de différentes dimensions, lesquelles contiennent le milieu de croissance ou des parties de celui-ci et sont stérilisées par les procédés standards de technologie pharmaceutique. De façon spécifique, ces formulations de pastilles sont appliquées pour contrôler une culture cellulaire de telle sorte que la phase d'adaptation est plus courte ou que la croissance est contrôlée par une libération de certains composants à un certain moment et dans une certaine concentration durant le procédé, et des nutriments (par exemple, de l'azote) peuvent être conditionnés dans le récipient de culture dans des quantités suffisantes pour des densités cellulaires élevées sans le risque d'intoxication de l'organisme.

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