Method for the tissue culture of a plant

Details Method for the tissue culture of a plant Method for the tissue culture of a plant

C - Chemistry, Metallurgy

12

N

195/47

C12N 5/04 (2006.01)

Patent

CA 1292961

72813-2 ABSTRACT OF THE DISCLOSURE A method for the tissue culture of a plant in a liquid medium, in which a phytohormone is fed to the medium intermittently under defined conditions to enable a stable culture for a long period with a high growth rate of the cell. The conditions include: (a) the feeding time interval, D(day), of the phytohormone is 0.5 to 5 times the doubling time of the cells, and (b) the amount of the phytohormone supplied satisfies the equation: Image where A is the amount (in moles) of the phytohormone fed intermittently to the medium; W is the fresh weight of the cells (in grams) present in the culture region; and D is as defined above.

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