Method for the treatment of diseased, degenerated, or...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/071 (2010.01) C12N 5/077 (2010.01) A61K 35/12 (2006.01) A61K 35/32 (2006.01) C12N 13/00 (2006.01)

Patent

CA 2473776

The invention relates to a tissue replacement structure, comprising (a) a pre- formed three-dimensional tissue which is produced from cells obtained from a human or animal organism, which are cultivated in cell culture vessels with hydrophobic surfaces and tapering base as a stationary suspension culture until a cell aggregate is achieved in which differentiated cells are embedded and which comprises an external region in which proliferation and migration cells are present, (b) (i) an autologous tissue cell suspension, produced from body cells with addition of body serum and without addition of growth- stimulating compounds, (ii) implants or support materials and/or (iii) growth factors and/or (c) the effect of electromagnetic fields, mechanical stimulation and/or ultrasound on the tissue from (a).

L'invention concerne une structure de remplacement tissulaire qui comprend : (a) un tissu tridimensionnel préformé, pouvant être obtenu de la façon suivante : on prélève des cellules dans l'organisme d'un homme ou d'un animal et on les met en culture, dans des récipients de culture cellulaire présentant une surface hydrophobe et un fond se rétrécissant, sous forme de culture en suspension, de façon fixe, jusqu'à l'obtention d'un agrégat cellulaire dans lequel des cellules différenciées sont incorporées et qui présente une zone extérieure dans laquelle se trouvent des cellules aptes à la prolifération et à la migration ; (b) (i) une suspension de cellules tissulaires autologue pouvant être obtenue à partir de cellules naturelles du corps avec addition de sérum naturel du corps et sans addition de composé stimulant la croissant, (ii) des implants ou des matériaux support et/ou (iii) des facteurs de croissance ; et/ou (c) le tissu selon (a) étant soumis à l'effet de champs électromagnétiques, d'une stimulation mécanique et/ou d'ultrasons.

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