Method for the treatment or diagnosis of human pathologies...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/0786 (2010.01) A61K 35/14 (2006.01) A61K 38/00 (2006.01) A61K 38/18 (2006.01) A61K 48/00 (2006.01) C12N 5/10 (2006.01) C12N 15/86 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2300387

Method for the treatment or diagnosis of pathologies either expressed in injured or pathological multiple sites in tissues or in the body or expressed in injured or pathological sites of tissues or cells in sites of the body, which are difficult to access, with said sites or areas in immediate proximity to said sites being the source of the release of chemotactic factors for endogenous macrophages, either spontaneously or upon suitable stimulation, wherein said treatment is carried out by administration to the body of an appropriate amount of exogenous monocyte derived cells, said monocyte derived cells being, in the case of treatment, loaded with corrective agents with respect to the pathologies to be treated, and with said monocyte derived cells having the properties of mobilisation towards the source of the above-said released chemotactic factors and to target the cells present in the vicinity of the said released chemotactic factors, and in the case of diagnosis, loaded with a marker enabling the detection of injured or pathological sites.

L'invention concerne une méthode de traitement ou de diagnostic d'affections exprimées soit dans des sites multiples pathologiques ou lésés de tissus ou du corps, soit dans des sites pathologiques ou lésés de tissus ou de cellules se trouvant dans des sites du corps difficiles d'accès. Ces sites ou zones se trouvent à proximité immédiate des sites sources de libération de facteurs chimiotactiques pour des macrophages endogènes, simultanément ou suite à une stimulation appropriée. On effectue ce traitement par administration dans le corps d'une quantité indiquée de cellules dérivées de monocytes exogènes, ces cellules dérivées de monocytes étant, en cas de traitement, chargées avec des agents correcteurs en fonction des affections à traiter, et ces cellules dérivées de monocytes possédant des propriétés de mobilisation vers la source des facteurs chimiotactiques libérés cités plus haut, et de ciblage des cellules présentes dans le voisinage des facteurs chimiotactiques libérés; et en cas de diagnostic, ces cellules dérivées de monocytes sont chargées avec un marqueur permettant la détection de sites pathologiques ou lésés.

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