Method for transferring gene

Details Method for transferring gene Method for transferring gene

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) C12N 15/87 (2006.01)

Patent

CA 2256496

A method of introducing a long-chain DNA molecule into a protoplast, comprising the step (a) of mixing a protoplast suspension, polyethylene glycol and a long-chain DNA molecule so as to achieve a final concentration of the DNA molecule of at least 50 µg/ml, the step (b) of harvesting the protoplasts, followed by washing and culturing thereof in a medium, and the step (c) of sorting the transformed cells. This method permits long-chain DNA molecules to be readily introduced into a wide variety of plant cells.

L'invention concerne un procédé d'introduction d'une molécule d'ADN à longue chaîne dans un protoplaste. Ce procédé consiste (a) à mélanger une suspension de protoplastes, un polyéthylèneglycol et une molécule d'ADN à longue chaîne de façon à obtenir une concentration finale de la molécule d'ADN d'au moins 50 µg/ml, (b) à récolter les protoplastes, puis à les laver et à les cultiver dans un milieu et (c) à trier les cellules transformées. Ce procédé permet aux molécules d'ADN à longue chaîne d'être facilement introduites dans une large variété de cellules de plantes.

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