Method for transformation of mono- and di- cotyledonous...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C12N 5/04 (2006.01) C12N 15/84 (2006.01) C12N 15/87 (2006.01)

Patent

CA 2451863

This invention relates to a method for the introduction of genes encoding desirable traits into both monocotyledonous and dicotyledonous plants and to plants and parts thereof produced by growing plants using this method. The time required for the production of transgenic plants is significantly decreased, while the number of transgenic plants is significantly increased. These increases are not dependent upon the use of super-virulent Agrobacterium strains. The invention also relates to an improved technique for in vitro regeneration of mono- and di-cotyledonous plants in a suitable medium containing a novel growth regulator regime that promotes cell elongation in the production of numerous somatic embryos that are regenerable into fertile plants.

La présente invention concerne une technique permettant d'introduire des gènes codant des traits recherchés dans des plantes monocotylédonées et dans des plantes dicotylédonées et des plantes et des parties de celles-ci produites par la culture de plantes utilisant cette technique. Le durée nécessaire à la production de plantes transgéniques est considérablement raccourcie, tandis que la production de ces plantes transgéniques est considérablement augmentée. Ces augmentations ne dépendent pas de l'utilisation de souches <i>Agrobacterium</i> super-virulentes. Cette invention concerne aussi une technique améliorée de régénération <i>in vitro</i> de plantes monocotylédonées et de plantes dicotylédonées dans un milieu adapté contenant un nouveau régime de régulateur de croissance qui favorise l'élongation cellulaire dans la production de nombreux embryons somatiques qu'on peut régénérer dans des plantes fertiles.

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