Method for transforming a cephalosporium cell

C - Chemistry – Metallurgy – 12 – N

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195/1.22, 195/1.

C12N 15/00 (2006.01) C12N 5/00 (2006.01) C12N 9/12 (2006.01) C12N 15/63 (2006.01) C12N 15/80 (2006.01)

Patent

CA 1261287

Abstract A method for transforming Cephalosporium and other lower eukaryotes is disclosed. The method involves inserting a recombinant DNA cloning vector comprising a Saccharomyces cerevisiae transcriptional and translational activating sequence positioned for expression of hygromycin phosphotransferase into a host cell and then growing the host cell under selective conditions. The vectors optionally further comprise a Cephalosporium-functional autonomous replication sequence, Cephalosporium ribosomal DNA, and also sequences that allow for replication and selection in E. coli and Streptomyces.

491351

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