Method for transforming monocotyledonous plants

Details Method for transforming monocotyledonous plants Method for transforming monocotyledonous plants

C - Chemistry, Metallurgy

12

N

C12N 15/87 (2006.01) A01H 4/00 (2006.01) A01H 5/00 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2104341

METHOD FOR TRANSFORMING MONOCOTYLEDONOUS PLANTS Abstract of the Disclosure A method for delivering foreign genetic material into immature embryos isolated from monocotyledonous plants, paticularly those of the Gramineae family is described. The method involves the isolation of immature embryos from the desired plant species, culturing the immature embryos for a period of time on a medium capable of inducing callus formation, introducing the foreign genetic material into the immature embryos and selecting transformed callus tissue to be regenerated into whole plants. Immature embryos that have undergone the culturing process prior to transformation exhibit a higher frequency of transformation when compared to that from freshly isolated immature embryos. The genetic material is preferably delivered by either electroporation or microprojectile bombardment methods.

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