Method for treating disseminated cancer

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/0783 (2010.01) A61K 35/14 (2006.01) A61K 39/00 (2006.01) A61P 35/00 (2006.01) A61K 38/20 (2006.01)

Patent

CA 2634169

The present invention discloses an immunotherapeutic method for treating a patient suffering from a disseminated cancer by administering expanded tumour- reactive CD4+ helper and/or CD8+ T-lymphocytes obtainable from one or more metastasis-draining lymph nodes (metinel nodes) draining a metastasis. The method comprises identification of one or more metinel lymph nodes in a patient, resection of the one or more nodes and, optionally all or part of the metastases, isolation of metastasis-reactive T-lymphocytes from said lymph nodes, in vitro expansion of said metastasis-reactive T-lymphocytes, and administration of the thus obtained T-lymphocytes to the patient, wherein the T-lymphocytes are CD4+ helper and/or CD8+ T-lymphocytes.

L'invention concerne une méthode immunothérapeutique permettant de traiter un patient souffrant d'un cancer disséminé par administration de lymphocytes T auxiliaires CD4+ et/ou de lymphocytes T CD8+ réactifs à une tumeur et ayant proliféré, pouvant être obtenus à partir d'un ou de plusieurs ganglions lymphatiques drainant une métastase (ganglions lymphatiques métastatiques). La méthode de l'invention consiste à identifier chez un patient un ou plusieurs ganglions lymphatiques métastatiques ; à pratiquer la résection du ou des ganglions et éventuellement d'une partie ou de la totalité des métastases ; à isoler les lymphocytes T réactifs à une métastase desdits ganglions lymphatiques ; à faire proliférerin vitro lesdits lymphocytes T réactifs à une métastase ; et à administrer les lymphocytes T réactifs à une tumeur ainsi obtenus au patient, lesdits lymphocytes T étant des lymphocytes T CD4+ auxiliaires et/ou des lymphocytes T CD8+.

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