C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 13/00 (2006.01) A61L 2/025 (2006.01) A61L 2/03 (2006.01) C02F 1/46 (2006.01) C02F 1/48 (2006.01) C12N 15/87 (2006.01)
Patent
CA 2388545
The invention relates to a method for treating an aqueous flux which is colonized by cells by means of a pulsed electric field which is applied to the flux, characterized in that the applied field is substantially parallel to the flow of the flux . The invention also relates to the use thereof in the transfer of nucleic acids (RNA,DNA, oligonucleotides) in cells, the transfer of proteins in cells, the extraction of molecules and cytoplasmic macromolecules contained in the cells, cell fusion and the production of hybrids and/or the insertion of membrane proteins. The invention further relates to an electropulsation chamber, a method for cellular destruction and a method for membrane permeabilization.
L'invention concerne un procédé de traitement d'un flux aqueux colonisé par des cellules par un champ électrique pulsé appliqué au flux caractérisé en ce que le champ appliqué est sensiblement parallèle à l'écoulement du flux et son application au transfert d'acides nucléiques (ARN, ADN, oligonucléotides) dans les cellules, au transfert de protéines dans les cellules, à l'extraction de molécules et de macromolécules cytoplasmiques contenues dans les cellules, à la fusion cellulaire et la production d'hybrides et/ou à l'insertion de protéines membranaires. Elle concerne en outre une chambre d'électropulsation, ainsi qu'un procédé de destruction cellulaire et un procédé de perméabilisation membranaire.
Cabanes Pierre-Andre Rene
Teissie Justin
Vernhes Marie-Christine
Centre National de La Recherche Scientifique
Fetherstonhaugh & Co.
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