Method for universal enzymatic production of bioactive peptides

C - Chemistry – Metallurgy – 12 – P

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C12P 21/06 (2006.01) C07K 14/605 (2006.01) C12N 9/52 (2006.01)

Patent

CA 2485695

The invention provides methods for making peptides from a polypeptide containing at least one copy of the peptide using clostripain to excise the peptide from the polypeptide. The methods enable the use of a single, highly efficient enzymatic cleavage to produce any desired peptide sequence.

L'invention concerne des méthodes d'obtention de peptides à partir d'un polypeptide renfermant au moins une copie du peptide, consistant à exciser le peptide du polypeptide à l'aide clostripaïne. Ces méthodes permettent d'obtenir n'importe quelle séquence peptidique à l'aide d'un seul clivage enzymatique de grande efficacité.

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