Method for use of branched nucleic acid probes

Details Method for use of branched nucleic acid probes Method for use of branched nucleic acid probes

C - Chemistry, Metallurgy

07

H

C07H 21/00 (2006.01) C12Q 1/44 (2006.01) C12Q 1/68 (2006.01) G01N 33/52 (2006.01) G01N 33/53 (2006.01) G01N 27/447 (2006.01)

Patent

CA 2128530

Nucleic acid hybridization probes having at least one nucleic acid strand which has at least two separate target specific regions (14, 16) that hybridize to a target nucleic acid sequence (15), and at least two distinct arm regions that do not hybri dize with the target nucleic acid but possess complementary regions that are capable of hybridizing with one another (18, 20). These regions are designed such that, under appropriate hybridization conditions, the complementary arm regions will not hybridize to one another in the absence of the target nucleic acid; but, in the presence of the target nucleic acid the target-specific regions of the probe will anneal to the target nucleic acid, and the complementary arm regions will anneal to one another (22), thereby forming a branched nucleic acid structure (21).

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