Method in the recovery of insulin

Details Method in the recovery of insulin Method in the recovery of insulin

A - Human Necessities

61

K

167/103.41

A61K 35/12 (2006.01) C07K 14/625 (2006.01)

Patent

CA 1041902

Abstract of the disclosure: A method in the recovery of insulin, particularly a method for the removal of fatty matter from an insulin and fatty matter containing pancreas extract. The extract, which may be the raw extract as separated from the residue of the comminuted pancreas glands after extraction, or an extract from which certain proteins different from insulin have been removed by precipitation by adjustment of the pH of the extract to about 8 and filtration, or any other extract containing insulin and fat, is cooled to a temperature at which all the fat freezes and separates out, whereupon the fat is separated from the extract, e.g. by filtration, under continued cooling. The extract can e.g. be cooled down to -30°C to -35°C. The crystallization of the fat can advantageously be carried out at a pH-value of about 3 under cautious agitation, the cooling time, until the final temperature of e.g. -30°C to -35°C is reached, being at least 2-3 hours.

231583

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