Method of accumulating allergen-specific t cell antigen...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/82 (2006.01) A01H 5/00 (2006.01) A23L 1/10 (2006.01) A23L 1/305 (2006.01) A61K 39/35 (2006.01) A61P 37/08 (2006.01) C07K 14/415 (2006.01) C12N 5/10 (2006.01) C12N 15/62 (2006.01)

Patent

CA 2523459

As techniques of accumulating a human T cell epitope in rice albumen, a method comprising accumulating a T cell epitope-attached peptide (7crp) directly in seeds, and a method comprising inserting 7crp into the variable region of glutelin, which is a major storage protein of rice, and expressing and accumulating the same as a part of the glutelin storage protein are developed. It is strongly expected that the rice capable of producing the T cell epitope- attached peptide thus developed would serve as a practically usable edible vaccine against cedar pollen allergy.

La présente invention a trait à des techniques d'accumulation de sites antigéniques de lymphocytes T humains dans l'albumine de riz, dont un procédé comprenant l'accumulation d'un peptide lié au site antigénique de lymphocyte T humain (7crp) directement dans des graines, et un procédé comprenant l'introduction de 7crp dans la région variable de glutéline, qui est une protéine importante de stockage du riz, et l'expression et l'accumulation de celui-ci comme partie intégrante de la protéine de stockage. Il est prévu que le riz capable de produire le peptide lié au site antigénique de lymphocyte T ainsi développé puisse être utilisé sous forme d'un vaccin comestible contre l'allergie au pollen de cèdre.

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