C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 7/02 (2006.01) C08F 8/12 (2006.01) C12N 7/04 (2006.01) C12N 15/10 (2006.01)
Patent
CA 2232622
A method of adsorbing from a solution comprising a biological sample viruses which retain their viability and infectivity. The method comprises adjusting the pH of said solution to pH 6.0 to 8.0; adding an effective amount of a water insoluble cross-linked polycarboxylic acid polymer ("WCPP") into said solution in a volume:volume ratio of WCPP to solution of 100:1 to 1:10,000 to form a WCPP-solution mixture; incubating said WCPP-solution mixture for a time sufficient to immobilize said viruses on said WCPP forming a WCPP-virus matrix; and separating said matrix from said solution. This novel method is suitable for removing, purifying, recovering and analyzing viable viruses as well as viral components such as viral proteins and nucleic acids.
L'invention se rapporte à un procédé d'adsorption, dans une solution comprenant un échantillon biologique, de virus conservant leur viabilité et leur infectivité. Ce procédé consiste à ajuster le pH de cette solution à un pH compris entre 6 et 8; à ajouter à cette solution une quantité efficace d'un polymère d'acide polycarboxylique réticulé, insoluble dans l'eau ("WCPP") dans un rapport volume:volume de WCPP et de solution compris entre 100:1 et 1:10000 afin d'obtenir un mélange WCPP-solution; à incuber ce mélange WCPP-solution sur une durée suffisante pour immobiliser ces virus sur ce WCPP formant une matrice WCPP-virus; et à séparer cette matrice de la solution. Ce nouveau procédé est également approprié pour éliminer, purifier, récupérer et analyser des virus viables ainsi que des composants viraux tels que des protéines et acides nucléiques viraux.
Arnold Edward
Donnelly Robert
Krupey John
Smith Allen D.
Arnold Edward
Donnelly Robert
Goudreau Gage Dubuc
Krupey John
Ligochem Inc.
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