A - Human Necessities – 01 – H
Patent
A - Human Necessities
01
H
A01H 5/00 (2006.01) A01H 5/10 (2006.01) C12N 5/04 (2006.01) C12N 15/29 (2006.01) C12N 15/82 (2006.01)
Patent
CA 2372793
The invention relates to a plant transformation method through treating germinating plant seeds with an Agrobaterium strain carrying a foreign DNA fragment. In particular, germinating plant seeds were used as receptors, Ti (tumor inducing) plasmid harbored in an Agrobacterium tumefaciens strain carrying an inserted foreign DNA fragment was used as a gene donor. The germinating plant seeds were co-cultured with the Agrobacterium strain, during the process the foreign DNA fragment was transferred into the donor plant genome. PCR amplification and PCR-Southern hybridization verified that the foreign gene, fragment has been transferred into the donor plant and can inherit to the next generation.
Li Yi
Liu Shaoxiang
Sun Yi
Wang Hui
Wang Jingxue
Robic
The Agri-Biotechnology Research Center Of Shanxi
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