Method of altering the binding specificity of proteins by...

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 16/00 (2006.01) A23C 9/152 (2006.01) A61K 35/14 (2006.01) A61K 39/395 (2006.01) B01D 57/02 (2006.01) C07K 1/107 (2006.01) C07K 1/113 (2006.01) C07K 2/00 (2006.01) C07K 16/18 (2006.01) C07K 16/40 (2006.01) G01N 33/564 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2602635

The binding specificity of at least one protein suspended or dissolved in a liquid medium is reversibly altered by exposing the protein to an oxidizing agent or an electric current. A masked protein such as an autoantibody can be detected, isolated and recovered from a biological fluid by subjecting the biological fluid to an oxidizing agent or an electric current to change the binding specificity of masked proteins contained therein.

Selon l'invention, la spécificité de liaison d'au moins une protéine en suspension ou en dissolution dans une substance liquide peut être modifiée de manière réversible par exposition de la protéine à un agent oxydant ou à un courant électrique. Une protéine masquée, comme un anticorps peut être détectée, isolée et récupérée à partir d'un fluide biologique par exposition du fluide biologique à l'action d'un agent oxydant ou à celle d'un courant électrique, afin de changer la spécificité de liaison des protéines masquées contenues dans le fluide biologique.

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