Method of amplifying hematopoietic stem cell and...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 5/00 (2006.01)

Patent

CA 2554192

Means useful in an amplification culture system of hematopoietic cells (hematopoietic stem cells and hematopoietic precursor cells). In particular, there are provided a composition for amplification of hematopoietic cells (hematopoietic stem cells and hematopoietic precursor cells), comprising a recombinant human serum albumin; and a non-serum culture medium for amplification of hematopoietic cells, comprising a fundamental culture medium and a recombinant human serum albumin. Further, there are provided a method of amplifying hematopoietic cells characterized in that hematopoietic cells are cultured in a non-serum culture medium containing a recombinant human serum albumin; and a hematopoietic culture obtained by this amplification method.

Cette invention concerne des moyens convenant pour un système de culture d'amplification de cellules hématopoïétiques (cellules souches hématopoïétiques et cellules précurseurs hématopoïétiques). L'invention concerne en particulier une composition pour amplification de cellules hématopoïétiques (cellules souches hématopoïétiques et cellules précurseurs hématopoïétiques) contenant une albumine de sérum humain de recombinaison ; et un milieu de culture non sérique pour amplification de cellules hématopoïétiques, comprenant un milieu de culture fondamentale et une albumine de sérum humain de recombinaison. L'invention concerne également une méthode d'amplification de cellules hématopoïétiques caractérisée en ce que lesdites cellules sont cultivées dans un milieu de culture contenant une albumine de sérum humain de recombinaison ; et une culture hématopoïétique obtenue par cette méthode d'amplification.

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