Method of amplifying nucleic acid

Details Method of amplifying nucleic acid Method of amplifying nucleic acid

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/00 (2006.01)

Patent

CA 2697532

The present invention provides a method for detecting a polymorphism or mutation in nucleic acid comprising a first phase to amplify or enrich for a sequence comprising a polymorphism or mutation and a second phase for detecting the polymorphism or mutation, wherein both phases are performed in the same reaction vessel.

La présente invention concerne un procédé de détection d'un polymorphisme ou d'une mutation dans un acide nucléique, comprenant une première phase consistant à amplifier une séquence contenant un polymorphisme ou une mutation ou à enrichir l'acide nucléique en ladite séquence et une seconde phase consistant à détecter le polymorphisme ou la mutation, les deux phases étant mises en uvre dans le même réacteur.

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