Method of amplifying nucleic acid

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C12N 15/09 (2006.01) C12N 15/11 (2006.01)

Patent

CA 2417798

A method of highly sensitively and specifically amplifying a target nucleic acid in a sample by using a chimeric oligonucleotide primer having a ribonucleotide provided at the 3'-terminus or in the 3'-terminal side, an endoribonuclease and a DNA polymerase having a chain transfer activity, i.e., an isothermal and chimeric primer-initiated amplification of nucleic acids (ICAN) method; a method of detecting an amplified fragment obtained by using the above method; a process for producing a target nucleic acid by using the above amplification method; and chimeric oligonucleotide primers to be used in these methods.

L'invention concerne un procédé d'amplification de manière particulièrement sensible et spécifique d'un acide nucléique cible dans un échantillon par utilisation d'une amorce oligonucléotidique chimère pourvu d'un ribonucléotide au terminus 3' ou du côté terminal 3', une endoribonucléase et un polymérase d'ADN ayant une activité de transfert de chaîne, c'est-à-dire un procédé d'amplification isothermique et chimérique initiée par amorce d'acides nucléiques (ICAN); un procédé de détection d'un fragment amplifié obtenu par utilisation du procédé susmentionné; une technique de production d'un acide nucléique cible par utilisation du procédé d'amplification susmentionné; et des amorces oligonucléotidiques chimères à utiliser dans ces procédés.

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