Method of analyzing enzyme

Details Method of analyzing enzyme Method of analyzing enzyme

C - Chemistry, Metallurgy

12

Q

C12Q 1/37 (2006.01) G01N 33/543 (2006.01) G01N 33/573 (2006.01)

Patent

CA 2609113

A method of analyzing an enzyme which comprises: (1) the step of contacting a test sample likely containing the target enzyme to be analyzed with an immobilized substrate, in which an enzyme substrate cleavable with the enzyme to be analyzed has been bound to an insoluble support, in a liquid; (2) the step of separating the insoluble support from the liquid; and (3) the step of analyzing the substrate or a fragment thereof remaining in the insoluble support and/or a fragment of the substrate released from the insoluble support into the liquid; and a kit for analyzing an enzyme containing at least an immobilized substrate in which an enzyme substrate cleavable with the enzyme to be analyzed has been bound to an insoluble support.

L~invention concerne un procédé d~analyse d~une enzyme qui comprend : (1) une étape consistant à mettre en contact un échantillon test renfermant vraisemblablement l~enzyme cible à analyser avec un substrat immobilisé, dans lequel un substrat d~enzyme clivable avec l~enzyme à analyser a été lié à un support insoluble, dans un liquide ; (2) une étape consistant à séparer le support insoluble du liquide ; et (3) l~étape consistant à analyser le substrat ou un fragment de ce dernier restant dans le support insoluble et/ou un fragment du substrat libéré du support insoluble dans le liquide ; et un kit destiné à analyser une enzyme contenant au moins un substrat immobilisé dans lequel un substrat d~enzyme clivable avec l~enzyme à analyser a été lié à un support insoluble.

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