Method of analyzing tumor cell dna content through tumor...

Details Method of analyzing tumor cell dna content through tumor... Method of analyzing tumor cell dna content through tumor...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12N 5/08 (2006.01) G01N 33/50 (2006.01) G01N 33/543 (2006.01) G01N 33/554 (2006.01) G01N 33/569 (2006.01) G01N 33/574 (2006.01) G01N 33/577 (2006.01)

Patent

CA 2160719

The invention provides a method for enriching a sample in tumor cells and determining the DNA ploidy and proliferative index of the tumor cells present in the sample. The method uses a pan-leukocyte monoclonal antibody conjugated to a separable substrate such as magnetic microspheres to conjugate and remove leukocytes from the sample. A fluorescently labelled monoclonal antibody specific to a tumor associated antigen or an antigen arising from the presence of the tumor and a DNA staining reagent are added to the leukocyte depleted sample. The sample is then analyzed by dual color flow cytometry to determine cells having an abnormal DNA content by gating on the fluorescently labelled cells.

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