Method of assay for antigen

Details Method of assay for antigen Method of assay for antigen

G - Physics

01

N

167/44

G01N 33/53 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2002470

A method of assay for antigen comprising the following processes (A), (B), (C) and (D): Process (A): A process in which the antigen to be assayed in the subject solution is bound with a functional group or marker to form a modified antigen. Process (B) : A process in which the modified antigen is bound to a carrier via an antibody against the antigen and then the carrier is separated from the subject solution. Process (C): The following process (a) or (b): (a) A process in which the modified antigen is diassociated from the carrier. (b) A process in which the modified antigen-antibody complex comprising the modified antigen and the antibody against the antigen is diassociated from the carrier. Process (D): A process in which the modified antigen or modified antigen-antibody complex of Process (C) is assayed. This method permits assay for antigens with high sensiti- vity based on characteristic features of the sandwich method, using an antibody against a single epitopic site, and also permits assay for low molecular substances, which have never been assayed by the conventional sandwich method, with higher sensitivity in comparison with the conventional competitive method.

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