Method of assay using magnetically held label

Details Method of assay using magnetically held label Method of assay using magnetically held label

G - Physics

01

N

324/23

G01N 33/53 (2006.01) C12M 1/40 (2006.01) G01N 33/532 (2006.01) G01N 33/543 (2006.01) G01N 33/553 (2006.01)

Patent

CA 1272507

ABSTRACT Method of Assay Using Magnetically Held Label In its broadest aspect, the present invention provides a method of effecting an electrochemical specific-binding assay of a ligand, either qualitatively or quantitatively, in an apparatus comprising at least one electrode, in which method a labelled component of the assay medium is, at least in part, magnetically held in the vicinity of the electrode. The electrochemical assay method may include a separation step, whereby bound label may be separated from free label in the assay medium. Preferably the electrochemical assay method will include the step of determining a perturbation in an electrochemical characteristic of components of the assay medium associated with a ligand complexing reaction. Such an assay method is applicable to, for example, antibodies and antigens and suitable labels include redox centres, enzyme labels in the presence of an electron-transfer mediator and electron-transfer mediator labels in the presence of an electron donor or acceptor.

486546

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