Method of assay using two distinguishable types of particles

Details Method of assay using two distinguishable types of particles Method of assay using two distinguishable types of particles

G - Physics

01

N

G01N 33/543 (2006.01) G01N 33/546 (2006.01)

Patent

CA 2179823

A binary assay method capable of providing wide dynamic range and a high degree of precision in which analyte and labelled ligand are reacted with two independently determinable forms of solid-supported binding partners having affinity for the analyte and labelled ligand respectively, the analyte concentration being determined from signals deriving from the resulting two forms by reference to a double standard calibration curve. A kit for use in the method is also disclosed.

L'invention se rapporte à une méthode de titrage binaire permettant d'obtenir un rayon d'action dynamique étendu et un haut niveau de précision, et dans lequel on fait réagir l'analyte et le ligand marqué avec deux formes indépendamment déterminables de partenaires de liaison placés en support sur un solide ayant une affinité respectivement pour l'analyte et pour le ligand marqué, la concentration de l'analyte étant déterminée à partir de signaux émanant des deux formes résultantes par référence à une double courbe d'étalonnage standard. L'invention se rapporte également à un kit utilisé dans ce procédé.

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