Method of assaying blood component by using whole blood and...

G - Physics – 01 – N

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G01N 33/66 (2006.01) C12Q 1/32 (2006.01) C12Q 1/48 (2006.01) C12Q 1/54 (2006.01) G01N 33/48 (2006.01)

Patent

CA 2614299

[PROBLEMS] In quickly assaying a blood component interfered by glucose and/or its derivative on the bedside or in a clinic or in assaying the same by a patient in his/her own home, there has been required an assay method wherein the whole blood can be used as a sample as such without resorting to a centrifuge or the like. [MEANS FOR SOLVING PROBLEMS] A method of assaying a blood component to be used for assaying a blood component interfered by glucose and/or its derivative, characterized by comprising bringing the whole blood into contact with a substance capable of converting glucose and/or its derivative into another substance not interfering the assay and, simultaneously or subsequently, separating blood cells; a device to be used in the assay method; and a kit containing this device.

Le problème à résoudre dans le cadre de cette invention réside en ce que, lors d~un test rapide d~un composant sanguin perturbé par du glucose et/ou son dérivé au chevet d~un patient ou dans un cabinet médical ou lors d~un test dudit composé par un patient à son domicile, un procédé de test est nécessaire dans lequel le sang total peut être utilisé tel quel comme échantillon sans avoir recours à une centrifugeuse ou analogue. La solution proposée consiste à prévoir un procédé de test d~un composant sanguin à utiliser pour tester un composant sanguin perturbé par du glucose et/ou son dérivé, caractérisé en ce qu~il comprend la mise en contact du sang total avec une substance capable de convertir le glucose et/ou son dérivé en une autre substance qui ne perturbe pas le test et à séparer, simultanément ou ultérieurement, les cellules sanguines ; à prévoir un dispositif à utiliser dans le procédé de test ; et un kit contenant ledit dispositif.

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