Method of assaying coenzymes a in biological sample

Details Method of assaying coenzymes a in biological sample Method of assaying coenzymes a in biological sample

G - Physics

01

N

G01N 30/88 (2006.01) G01N 27/62 (2006.01) G01N 30/04 (2006.01) G01N 30/72 (2006.01) G01N 33/573 (2006.01) G01N 30/06 (2006.01)

Patent

CA 2480306

It is intended to provide a highly sensitive and highly reproducible method of assaying coenzymes A (CoA) in a biological sample characterized by involving the step of extracting the biological sample by using a strongly acidic solution, the step of solid phase extraction, the step of adding an internal standard, and the step of detecting with the use of LC-MS.

L'invention concerne une technique hautement sensible et hautement reproductible de dosage de coenzymes A (CoA) dans un échantillon biologique caractérisée en ce qu'elle comprend une opération d'extraction de l'échantillon biologique au moyen d'une solution fortement acide, une opération d'extraction en phase solide, une opération d'ajout d'un étalon interne, et enfin, une opération de détection faisant intervenir LC-MS.

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