Method of assaying oxidized lipoprotein and application thereof

Details Method of assaying oxidized lipoprotein and application thereof Method of assaying oxidized lipoprotein and application thereof

G - Physics

01

N

G01N 33/543 (2006.01) G01N 33/566 (2006.01) G01N 33/92 (2006.01)

Patent

CA 2172247

Use of an anti-cardiolipin antibody, anti- lipoprotein antibody or anti-.beta.2-glycoprotein I antibody together with an immobilized antibody thereof enables to accurately assay for a complex of .beta.2-glycoprotein I and an oxidized lipoprotein in a blood sample, accord- ing to a sandwich immunoassay. Thus, the oxidized lipoprotein in blood can be detected, whereby diagnosis of arteriosclerotic disease is enabled.

Procédé permettant d'effectuer, à l'aide d'une glycoprotéine .beta.2 I, un titrage précis d'un complexe de lipoprotéine oxydée dans un échantillon sanguin par une technique sandwich, consistant à utiliser un anticorps anticardiolipine, un anticorps antilipoprotéine ou un anticorps antiglycoprotéine .beta.2 I et un dérivé immobilisé de celui-ci. Ce procédé permet de détecter une lipoprotéine oxydée dans le sang et d'établir le diagnostic de maladies artériosclérotiques.

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