Method of assaying the presence of cancer cells

Details Method of assaying the presence of cancer cells Method of assaying the presence of cancer cells

G - Physics

01

N

167/37

G01N 33/574 (2006.01) C07K 16/30 (2006.01) G01N 33/577 (2006.01)

Patent

CA 1271418

METHOD OF ASSAYING THE PRESENCE OF CANCER CELLS ABSTRACT Method to detect and/or determine the presence of cancer cells in mammals, including humans, and to monitor the progress of treatment for cancer. A human protein marker, the assay-marker, has been ascertained of the molecular weight 70,000-74,000, which is secreted by cancer cells at levels 10-fold or greater than that observed with normal cells. An assay is described where the assay-marker or an antigenically analogous protein, the analog-marker, is used to prepare antibodies to the assay-marker. The antibodies are then reacted with blood or serum samples to determine the level of the assay-marker in the sample. Thus, the assay essentially comprises (1) providing an antibody to the protein described above (2) reacting the antibody of step 1 with the sample to be tested and (3) measuring the level of the reacted antibody to detect and/or determine the presence and/or quantity of cancer cells.

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