Method of attachment of a biomolecule to a solid surface

Details Method of attachment of a biomolecule to a solid surface Method of attachment of a biomolecule to a solid surface

C - Chemistry, Metallurgy

09

D

C09D 183/04 (2006.01) B01J 19/00 (2006.01) C08L 83/00 (2006.01) C12M 1/34 (2006.01) C12Q 1/68 (2006.01) G01N 33/48 (2006.01) G01N 33/543 (2006.01) G01N 33/552 (2006.01)

Patent

CA 2460528

Disclosed is a method for attaching biomolecules to a solid surface and a composition for preparing that surface for attachment. The composition comprises molecules of Formula I and Formula II which are defined as follows: Y - X - Z - R1 Formula I Y'- X' - Z' - R2 Formula II and wherein R1 is a biomolecule, a reactive group or a group capable of forming a reactive group; R2 is different to R1 and is present in at least a 104 fold molar excess to R1;Y and Y' are groups which can bind to a solid surface; X and X' are atoms which are, at least, bivalent; and Z and Z' are linker groups.

L'invention concerne un procédé de fixation de biomolécules à une surface et une composition permettant de préparer cette surface à la fixation. La composition comprend des molécules de formule I: Y - X - Z - R1, et de formule II : Y'- X' - Z' - R2; dans lesquelles R1 est une biomolécule, un groupe réactif ou un groupe capable de former un groupe réactif; R2 est différent par rapport à R1 et est présent en excès molaire d'au moins 10?4¿ fois par rapport à R1; Y et Y' sont des groupes qui peuvent se fixer à une surface solide; X et X' sont des atomes qui sont, au moins, bivalents; et Z et Z' sont des groupes de liaison.

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