Method of attenuating viruses

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/70 (2006.01) C12N 5/00 (2006.01) C12N 7/00 (2006.01) C12N 7/02 (2006.01) G01N 33/566 (2006.01) A61K 39/12 (2006.01) A61P 31/12 (2006.01)

Patent

CA 2263993

The present invention provides a method of selecting virus vaccine candidates by selecting virus variants that do not bind to brain membrane receptor preparations, comprising the steps of: (a) preparing a brain membrane receptor preparation; (b) mixing an amount of a virus of interest with an amount of said membrane receptor preparation containing excess membrane receptors to form a virus-membrane receptor preparation suspension; (c) centrifuging said suspension to form a supernatant; (d) determining residual virus infectivity in said supernatant; and (e) isolating individual membrane receptor preparation binding-resistant virus variants which are useful as virus vaccine candidates.

L'invention porte sur un procédé de sélection de candidats de virus utilisables comme vaccins consistant à sélectionner des variantes de virus ne se fixant pas sur les préparations de récepteurs de membrane cérébrale. Ledit procédé consiste à: (a) préparer une préparation de récepteurs de membrane cérébrale; (b) mélanger une quantité du virus d'intérêt avec une quantité de la susdite préparation contenant un excès de récepteurs de membrane pour obtenir une suspension de virus et de récepteurs de membrane; (c) centrifuger ladite suspension pour former un surnageant; (d) déterminer le pouvoir infectieux résiduel du surnageant; (e) isoler les variantes individuelles de virus résistant à la fixation sur la préparation de récepteurs de membrane.

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