Method of breeding lipid-producing fungus

Details Method of breeding lipid-producing fungus Method of breeding lipid-producing fungus

C - Chemistry, Metallurgy

12

N

C12N 1/15 (2006.01) C12N 15/00 (2006.01)

Patent

CA 2536606

It is intended to provide a breeding method whereby a lipid-producing fungus belonging to the genus Mortierella can be effectively and efficiently bred without resort to antibiotics. Transformation is carried out with the use of an auxotroph (for example, uracil auxotroph) of a fungus belonging to the genus Mortierella as a host. More specifically speaking, a gene compensating for the auxotrophy, which is employed as a marker gene, is transferred into the host (the gene transfer step). Next, a transformant is selected by using the recovery of the auxotrophy as the marker (the selection step). As an example of the lipid-producing fungus as described above, M. aplina may be cited.

L'invention concerne un procédé de culture au moyen duquel un champignon producteur de lipides du genre Mortierella peut être effectivement et efficacement cultivé, sans utilisation d'antibiotiques. La transformation s'accomplit par la mise en oeuvre d'un auxotrophe (par exemple l'auxotrophe pour l'uracile) d'un champignon du genre Mortierella utilisé comme hôte. Plus particulièrement, un gène compensateur de l'auxotrophie, utilisé comme gène marqueur, est transféré dans l'hôte (étape de transfert des gènes). Un transformant est ensuite sélectionné par récupération de l'auxotrophie en tant que marqueur (étape de sélection). M. aplina peut être cité comme exemple du champignon producteur de lipides de l'invention.

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