Method of cell cultures and device for implementing it

Details Method of cell cultures and device for implementing it Method of cell cultures and device for implementing it

C - Chemistry, Metallurgy

12

M

C12M 3/00 (2006.01) C12M 1/02 (2006.01) C12M 1/40 (2006.01)

Patent

CA 2624729

Cell culture device (1) comprising a culture vessel (2) provided with a cover (19) in which there is a first zone (3) for transferring culture medium essentially free from cells internal to a second cell culture zone (5), a third (4) and a fourth zone (6) for transferring culture medium essentially free from cells. The third zone (4) is internal to the second zone (5) and external to the first zone (4) and the fourth zone (6) being a zone external to said second zone (5). All the zones are in medium communication. The device also comprises culture medium circulation means, allowing a circulation of the culture medium through the culture zone (5) from bottom to top.

L'invention concerne un dispositif de culture cellulaire (1) qui comprend une cuve de culture (2) munie d'un couvercle (19) dans laquelle sont ménagées une première zone (3) utilisée pour transférer un milieu de culture sensiblement exempt de cellules qui est située à l'intérieur d'une deuxième zone de culture cellulaire (5), une troisième (4) et une quatrième zone (6) permettant de transférer un milieu de culture sensiblement exempt de cellules. La troisième zone (4) est située à l'intérieur de la deuxième zone (5) et à l'extérieur de la première zone (4), et la quatrième zone (6) est située à l'extérieur de la deuxième zone (5). Toutes les zones sont en communication de façon à assurer un transfert du milieu. Le dispositif de l'invention comprend également un moyen de circulation de milieu de culture qui permet de faire circuler ce dernier dans la zone de culture (5) de bas en haut.

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