Method of cleansing proteins

Details Method of cleansing proteins Method of cleansing proteins

C - Chemistry, Metallurgy

07

K

C07K 1/14 (2006.01) C07K 1/34 (2006.01) C07K 14/76 (2006.01) C07K 16/00 (2006.01) C07K 16/06 (2006.01)

Patent

CA 2058958

The invention relates to a method of cleansing a protein from multivalent metal ions bound thereto, these ions being released from the protein by exchanging the ions with monovalent metal ions, whereafter the multivalent metal ions are removed. The release and removal of these ions is effected, in particular, by diafiltration or gel filtration processes.

L'invention concerne un procédé de purification d'une protéine des ions métalliques multivalents liés à cette cible, ces ions étant libérés de la protéine par échange des ions par des ions métalliques monovalents, après quoi les ions métalliques multivalents sont éliminés. La libération et l'élimination de ces ions s'effectuent, notamment, selon des procédés de diafiltration ou de filtration par gel.

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