Method of cloning mammalian embryos

Details Method of cloning mammalian embryos Method of cloning mammalian embryos

C - Chemistry, Metallurgy

12

N

C12N 5/16 (2006.01) C12N 5/08 (2006.01) C12N 5/10 (2006.01)

Patent

CA 2043088

Method of Cloning Mammalian Embryos ABSTRACT OF THE DISCLOSURE An improved method of cloning mammalian embryos by transferring a nucleus from a donor mammalian embryo to an enucleated recipient oocyte and culturing the nuclear transferred bovine embryo in vitro is described. The improvement includes positioning the donor embryo nucleus adjacent to the enucleated recipient oocyte, and maintaining the nucleus and oocyte in a maintenance medium for a time sufficient to increase the intercellular surface area contact between the donor membrane-bounded nucleus and the enucleated recipient oocyte prior to fusing the donor nucleus to the recipient oocyte.

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