Method of cloning modified streptomycetes dna

Details Method of cloning modified streptomycetes dna Method of cloning modified streptomycetes dna

C - Chemistry, Metallurgy

12

N

195/1.16, 195/1.

C12N 15/00 (2006.01) C12N 1/20 (2006.01) C12N 15/63 (2006.01) C12N 15/76 (2006.01)

Patent

CA 1251748

Abstract A method of cloning endogenously modified Streptomycetes DNA, which is normally rejected by restrictionless heterospecific hosts, is disclosed. The method uses bacteriophage lambda to construct a genomic library of modified Streptomycetes DNA; such lambda- containing Streptomycetes DNA is replicated to provide a source of non-modified Streptomycetes DNA. This non- modified DNA is subcloned into a selectable cloning vector and used to transform restrictionless hetero- specific hosts. The transformants can then be screened for clones containing genes of interest.

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