Method of collecting highly pure polyhydroxyalkanoate from...

C - Chemistry – Metallurgy – 12 – P

Patent

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C12P 7/62 (2006.01)

Patent

CA 2512031

It is intended to provide a method of separating and purifying PHA whereby cell constituents other than PHA particles can be efficiently removed from PHA- containing microbial cells and thus highly pure PHA can be obtained at a high yield without causing any serious decrease in molecular weight, and a method of obtaining PHA grain aggregates. Namely, a method of collecting PHA which comprises subjecting an aqueous suspension of PHA-containing microbial cells to a mechanical disruption treatment at a low temperature and adding an alkali to thereby efficiently disrupt the microbial cells and, after collecting PHA, treating with an enzyme and/or a surfactant. Moreover, the PHA particle size can be enlarged by suspending the PHA in a hydrophilic solvent and/or water and stirring the suspension at a temperature lower than its boiling point to thereby induce aggregation.

L'invention concerne un procédé de séparation et de purification de polyhydroalcanoate (PHA), permettant d'éliminer efficacement les constituants cellulaires autres que les particules de PHA des cellules microbiennes contenant du PHA, ce qui permet d'obtenir un PHA présentant une pureté élevée, avec un haut rendement, sans entraîner de diminution importante du poids moléculaire, et un procédé permettant d'obtenir des agrégats de granules de PHA. L'invention concerne notamment un procédé de collecte de PHA qui consiste à soumettre une suspension aqueuse de cellules microbiennes contenant du PHA à un traitement de dissociation mécanique à basse température, et à ajouter un alcali afin de provoquer une dissociation efficace des cellules microbiennes, et après avoir collecté le PHA, à appliquer un traitement avec un enzyme et/ou un tensioactif. La taille des particules de PHA peut en outre être accrue par mise en suspension du PHA dans un solvant hydrophile et/ou de l'eau, et agitation de la suspension à une température inférieure à son point d'ébullition de manière à provoquer l'agrégation.

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