Method of compensating toxicity test data for the measured...

Details Method of compensating toxicity test data for the measured... Method of compensating toxicity test data for the measured...

C - Chemistry, Metallurgy

12

Q

C12Q 1/18 (2006.01) C12Q 1/66 (2006.01) G01N 21/76 (2006.01)

Patent

CA 2131624

2131624 9319198 PCTABS00025 A method for compensating toxicity test data for the measured toxicity of a reference sample consists of the steps of: a) obtaining toxic response data for a plurality of concentrations of at least two different test samples, including reagent controls having zero sample concentration for each sample; b) defining the sample having the smallest toxic response at high concentrations as the reference sample; c) computing the toxic response of the test sample relative to that for the reference sample for at least two concentrations in accordance with the equation: relative gamma = .GAMMA.R = (LSC/LSX)(LRX/LRC)-1, where LSC = light output of the test sample control (i.e. for X = 0), LSX = light outputof the test sample for concentration X, LRX = light output of the reference sample for concentration X, LRC = light output of the reference sample control (i.e. for X = 0); and d) generating a dose-response curve of concentration versus relative .GAMMA. (gamma) for at least two corresponding concentrations of the two samples.

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