C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
150/16.4, 73/51.
C12Q 1/06 (2006.01) C12Q 1/68 (2006.01) G01N 21/64 (2006.01)
Patent
CA 1201963
Method of Concentrating and Measuring Unicellular Organisms Abstract A method of concentrating and measuring unicellular organism content of a sample is described. The unicellu- lar organism containing sample is first filtered through a filter membrane impermeable to the organism to con- centrate and collect the cells on the filter membrane. The membrane containing the concentrated cells is then sequentially treated (1) with a lysing agent to lyse the cells releasing the adenosine triphosphate in the cells and (2) with a luminescent reagent which reacts with the released adenosine triphosphate contained on the filter to produce light. Without significant effect by the filter membrane, the light produced on the mem- brane is then measured on a luminometer. Not only does this method provide an accurate way of detecting very minute quantitites of cells, but it provides a rela- tively quick and easy method of concentrating and measuring cells in large volumes of biological or industrial liquid samples which contain low levels of organisms.
432916
Ogilvy Renault
Packard Instrument Company Inc.
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