Method of concentrating shear-sensitive biopolymers using...

B - Operations – Transporting – 01 – D

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B01D 61/24 (2006.01) A61M 1/16 (2006.01) C07K 1/34 (2006.01) C07K 14/745 (2006.01)

Patent

CA 2732790

The invention relates generally to methods of concentrating mixtures including shear sensitive biopolymers, such as von Willebrand Factor. Conventional methods of concentrating biopolymers impart too much shear stress, which causes the degradation of shear sensitive biopolymers. The methods disclosed herein reduce the shear stress while maintaining a high rate of filtrate flux. Disclosed herein is a method for concentrating shear sensitive biopolymers including flowing a mixture with a shear sensitive biopolymer into a hollow fiber dialysis module to form a retentate having a shear sensitive biopolymer concentration that is greater than that of the mixture. Hollow fiber dialysis modules have high filtrate fluxes and low shear rates at low flow rates. This ensures a high product yield and minimal loss of shear sensitive biopolymers.

Linvention porte en général sur des procédés permettant la concentration de mélanges comprenant des biopolymères sensibles au cisaillement, notamment au facteur de Von Willebrand. Les procédés classiques de concentration de biopolymères impriment une contrainte de cisaillement trop importante, qui provoque la dégradation des biopolymères sensibles au cisaillement. Les procédés ici décrits permettent de réduire la contrainte de cisaillement tout en maintenant un taux élevé de flux de filtrat. La présente invention concerne un procédé de concentration de biopolymères sensibles au cisaillement comprenant lécoulement dun mélange contenant des biopolymères sensibles au cisaillement dans un module de dialyse à fibres creuses pour former un rétentat possédant une concentration en biopolymères sensibles au cisaillement supérieure à celle du mélange. Les modules de dialyse à fibres creuses présentent des flux de filtrat élevés et des taux de cisaillement bas à faibles débits. Ceci permet dassurer un rendement élevé du produit et une perte minimale en biopolymères sensibles au cisaillement.

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