Method of constructing and screening libraries of peptide...

C - Chemistry – Metallurgy – 40 – B

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C40B 40/10 (2006.01)

Patent

CA 2638912

The present invention provides the means for producing libraries of peptide structures for drug screening applications that are capable of folding or assuming their native conformations independently of artificial scaffolds or flanking sequences in the proteins from which they are derived. The libraries can be highly diverse such that they are representative of the repertoire of protein structures existing in nature. The libraries can also be non-redundant or normalized such that the bias towards specific structures existing in source data sets and/or in nature is/are removed. In a particularly preferred embodiment, the present invention provides 30,000 independent fold structures produced by this method. The present invention also provides computer - readable media and systems comprising structural data in relation to the peptide libraries, and methods for displaying and screening the libraries.

La présente invention concerne les moyens de produire des bibliothèques de structures peptidiques pour des applications de dépistage de drogues capables de plier ou d'adopter leurs conformations natives indépendamment de charpentes artificielles ou de régions flanquantes dans les protéines desquelles elles proviennent. Les bibliothèques peuvent être très variées de manière à représenter le répertoire des structures des protéines existant dans la nature. Les bibliothèques peuvent également être non répétitives ou normalisées de telle sorte que la ou les déviations vers des structures spécifiques existant dans les ensembles de données source et/ou dans la nature est/sont éliminées. Dans un mode de réalisation particulièrement préféré, la présente invention propose 30 000 structures pliées indépendantes produites par ce procédé. L'invention concerne également des supports pouvant être lus par ordinateur et des systèmes comprenant des données structurales en relation avec les bibliothèques de peptides, ainsi que des procédés d'affichage et de criblage des bibliothèques.

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