Method of constructing masses of myocardial cells and use of...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/077 (2010.01) A61K 35/34 (2006.01)

Patent

CA 2690610

The object of the present invention is to improve the post-transplantation engraftment rate of cardiomyocytes that have been purified to such an extent that they are free from non-cardiomyocytes and any components derived from other species. To solve this problem, the present inventors studied the possibility of constructing cell masses from the purified cardiomyocytes. As a result, they revealed that the stated problem could be solved by providing a method of preparing cell masses of cardiomyocytes derived from pluripotent stem cells, characterized in that cell masses of aggregated cells containing cardiomyocytes that had been differentiated and induced from pluripotent stem cells were dispersed to single cells to thereby obtain purified cardiomyocytes, which were then cultured in a culture medium under serum-free conditions so that they were reaggregated.

L'invention vise à proposer une vitesse de prise de post- transplantation de cellules myocardiques qui ont été purifiées de telle sorte qu'elles sont composées exclusivement de cellules myocardiques et exemptes de cellules issues dans d'autres espèces. Pour résoudre le problème ci-dessus, il a été discuté si ou non une masse cellulaire de cellules myocardiques purifiées peut être construite. Comme résultats, il a été clarifié que le problème décrit ci-dessus peut être résolu par la fourniture d'un procédé pour construire une masse de cellules myocardiques issues de cellules souches pluripotentes caractérisé par le fait qu'il comprend la dissociation d'une masse cellulaire agrégée contenant des cellules myocardiques, qui ont été différenciées et sont issues de cellules souches pluripotentes, pour donner des cellules uniques individuelles, l'incubation des cellules myocardiques issues de cellules souches pluripotentes ayant ainsi été purifiées par l'utilisation d'un milieu dans des conditions exemptes de sérum et ainsi s'agréger aux cellules.

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